New AAV Production Method

Coming Soon!!

As with all vector systems, AAV systems have disadvantages. The greatest obstacle in rAAV production is scalibility. Most rAAV production methods are very cumbersome and time-consuming because of the number of cells that need to be transduced. Because it is extremely difficult to produce large quantities of high titer rAAV, animals studies and clinical studies have been next to impossible to carry-out.

Another disadvantage of rAAV production, is the purification step. The classical method for purification is ultracentrifugation on a cesium chloride or iodixanol gradient. These methods of purification are limited by the capacity of cell lysate volume that can be processed at one time and by the low purity of the final product. Despite these disadvantages, these methods are still widely used for basic research and small-scale animal studies.

To address these disadvantages, we developed a New rAAV Production System that can be easily scaled up for production of large quantities of highly purified rAAV. Unlike some of the more recently developed methods for rAAV production, our method promises to provide high titer virus that requires minimal processing between production and purification. Additionally, with our system, you do not need to worry about your vector being produced in a non-mammalian cell line. Our system offers all the advantages of using a mammalian system including, appropriate post-translational modification and presence of the proper chaperones for aiding in molecular folding.

With the new rAAV vector production method, we will also implement a purification method using FPLC. FPLC purification of rAAV yields highly pure virus with minimal loss of virus.

We will be offering this new service in the near future so check back for updates and a date when our new rAAV production method will be available.

To request an AAV service, visit our Initate AAV Service page or fill out and fax your Service Request Form to us at 713-798-1230 or e-mail to vector@bcm.tmc.edu.