Standard Operating Procedures - Vector Development Laboratory

Standard Operating Procedures

All vectors produced by the VDL are manufactured in compliace with Good Laboray Practices (GLP). Our committment to GLP regulations makes all of our vectors suitable to pre-clinical studies. There are many features that constitute compliance with GLP including establishing and strictly following Standared Operating Procedures (SOPs) for the production of all vectors. Below you will find links to all SOPs followed by the VDL. Feel free to check out our SOPs and contact us if you have questions.

Adenoviral Vector SOPs
VDL100.1 Cloning transgene into pAdenoX
VDL101.2 Cloning transgene into pAd5F35
VDL102.2 Production of adenoviral vector in 293 cells (transfection)
VDL103.2 Expansion and harvesting plaque purified adenoviral vector
VDL104.2 Extraction of adenoviral DNA and genome analysis (HIRT DNA)
VDL105.1 Preparation of lysate
VDL106.2 Large-scale expansion and purification of adenoviral vector

Adeno-Associated Viral Vector SOPs
VDL200.1 Production and large-scale of AAV vectors in 293 cells
VDL201.1 Purification of AAV vectors

Retroviral Vector SOPs
VDL300.2 Production of retrovirus by transient transfection

Lentiviral Vector SOPs
VDL400.1 Production of lentiviral vectors in 293FT cells

Plasmid Production SOPs
VDL500.1 Large-scale production of plasmid DNA
VDL501.1 Small-scale production of plasmid DNA

Quality Assurance/Quality Control SOPs
VDL600.1 Rapid assay for detection of replication competent adenovirus
VDL601.2 Standard assay for detection of replication competent adenovirus
VDL602.1 Rapid assay for adenovirus titration (pfu/ml)
VDL603.1 Plaque assay for adenovirus titration (pfu/ml)
VDL604.1 Rapid assay for detection of replication competent AAV
VDL605.1 Replication center assay for determining AAV infectivity
VDL606.1 Rapid assay for quantitation of genome copies
VDL607.1 Assay for determining retrovirus infectivity
VDL608.1 Detection of replication competent retrovirus
VDL609.1 Assay for determining lentivirus vector
VDL610.1 Detection of replication competent lentivirus
VDL611.1 Test for sterility
VDL612.1 Test for endotoxin
VDL613.1 Test for cellular DNA
VDL614.1 Test for mycoplasma

Suport SOPs
VDL701.1 Growth and maintenance of 293FT cells
VDL702.1 Growth and maintenance of 293 cells
VDL703.1 Growth and maintenance of Phoenix A/E cells
VDL704.1 Phenol/Chloroform/Isoamyl Alcohol Extraction of DNA
VDL705.1 Growth and maintenance of A549 cells

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©2002, CAGT Vector Development Laboratory, Baylor College of Medicine
One Baylor Plaza, N1010, Houston, TX 77030, (713)798-1238
URL: http://vector.bcm.tmc.edu Email:vector@bcm.tmc.edu
(Modified: April 16, 2007 FC)



	Technical Information / Standard Operating Procedures / Cloning Vectors Standard Operating Procedures All vectors produced by the VDL are manufactured in compliace with Good Laboray Practices (GLP). Our committment to GLP regulations makes all of our vectors suitable to pre-clinical studies. There are many features that constitute compliance with GLP including establishing and strictly following Standared Operating Procedures (SOPs) for the production of all vectors. Below you will find links to all SOPs followed by the VDL. Feel free to check out our SOPs and contact us if you have questions. Adenoviral Vector SOPs VDL100.1 Cloning transgene into pAdenoX VDL101.2 Cloning transgene into pAd5F35 VDL102.2 Production of adenoviral vector in 293 cells (transfection) VDL103.2 Expansion and harvesting plaque purified adenoviral vector VDL104.2 Extraction of adenoviral DNA and genome analysis (HIRT DNA) VDL105.1 Preparation of lysate VDL106.2 Large-scale expansion and purification of adenoviral vector Adeno-Associated Viral Vector SOPs VDL200.1 Production and large-scale of AAV vectors in 293 cells VDL201.1 Purification of AAV vectors Retroviral Vector SOPs VDL300.2 Production of retrovirus by transient transfection Lentiviral Vector SOPs VDL400.1 Production of lentiviral vectors in 293FT cells Plasmid Production SOPs VDL500.1 Large-scale production of plasmid DNA VDL501.1 Small-scale production of plasmid DNA Quality Assurance/Quality Control SOPs VDL600.1 Rapid assay for detection of replication competent adenovirus VDL601.2 Standard assay for detection of replication competent adenovirus VDL602.1 Rapid assay for adenovirus titration (pfu/ml) VDL603.1 Plaque assay for adenovirus titration (pfu/ml) VDL604.1 Rapid assay for detection of replication competent AAV VDL605.1 Replication center assay for determining AAV infectivity VDL606.1 Rapid assay for quantitation of genome copies VDL607.1 Assay for determining retrovirus infectivity VDL608.1 Detection of replication competent retrovirus VDL609.1 Assay for determining lentivirus vector VDL610.1 Detection of replication competent lentivirus VDL611.1 Test for sterility VDL612.1 Test for endotoxin VDL613.1 Test for cellular DNA VDL614.1 Test for mycoplasma Suport SOPs VDL701.1 Growth and maintenance of 293FT cells VDL702.1 Growth and maintenance of 293 cells VDL703.1 Growth and maintenance of Phoenix A/E cells VDL704.1 Phenol/Chloroform/Isoamyl Alcohol Extraction of DNA VDL705.1 Growth and maintenance of A549 cells
	CAGT Home \| BCM Home \| Privacy Notices ©2002, CAGT Vector Development Laboratory, Baylor College of Medicine One Baylor Plaza, N1010, Houston, TX 77030, (713)798-1238 URL: http://vector.bcm.tmc.edu Email:vector@bcm.tmc.edu (Modified: April 16, 2007 FC)